SCIE-PLAS == Isoelectric Focusing

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Isoelectric Focusing

Isoelectric Focusing (IEF) is an ingenious process for the simultaneous concentration and separation of proteins. IEF employs a pH gradient formed by small amphoteric molecules (ampholytes) to resolve proteins according to their different pI (isoelectric point) values - i.e. the pH at which a protein has no net charge. IEF is an end-point method; when the electrophoresis run is completed proteins will appear as separate sharp zones, in the order of their pI. Scie-Plas is delighted to offer the following SERVA products for IEF, using immobilised pH gradient (IPG) strips, capillary ampholyte gels or precast IEF gels.

Ampholytes for Capillary Gel IEF

SERVALYT™ Carrier Ampholytes are low molecular weight molecules of zwitterioni...

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SERVA Precast Gels for Isoelectric Focusing

With SERVALYT™ PRECOTES™ precast gels, the days of elaborate, inconvenient casting of gels are gone. SERVA offer...

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SERVALYT™ PRECOTES™ STARTER KIT

The SERVALYT™ PRECOTES™ Starter Kit contains three SERVALYT™ PRECOTES™ pH 3-10 standard precast gels (125 x 125 x 0.25mm), elect...

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SERVA Liquid Mix IEF Marker pH 3-10

SERVA Liquid Mix IEF Marker pH 3-10 is a ready-to-use protein marker developed especially for IEF applications. The marker is applicable to all horizontal and vertical IEF gels and can be used in both native and denaturing conditi...

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Applicator Strips

Four different silicone applicator strips for loading sample on to SERVALYT™ PRECOTES gels.

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Electrode Wicks

Available in three sizes: -

Standard - 120 x 6 x 1mm

Long - 240 x 6 x 1mm

Extra Long - 300 x 6 x 1mm

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Electrode Fluid

Anode Fluid, pH3 - 0.17g L-aspartic acid / 0.18g L-glutamic acid in water Cathode Fluid, pH10

Cathode Fluid, pH10 - 0.22g L-arginine, 0.18g L-lysine, 6.0ml ethylenediamine in water.

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Bayol F and Kerosene Pure

Bayol F and Kerosene Pure maintain adequate heat dissipation, which helps to prevent desiccation of IEF gels and IPG strips during IEF.

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SERVA IPG BlueStrips

SERVA IPG BlueStrips are dried gel strips, incorporating an immobilised pH gradient, used in high resolution 2-D gel electrophoresis of proteins. Ideal for...

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Rehydration Tray

To rehydrate up to 12 IPG strips of lengths up to 24cm. The rehydration tray maintains its shape and is resistant against the chemicals normally used to rehydrate IPG strips.

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