SERVALYTâ„¢ Carrier Ampholytes

SERVALYT™ Carrier Ampholytes are low molecular weight molecules of zwitterionic character. They are a mixture of synthetically derived species with an average molecular weight distribution of 400 to 1000 Da, and comprise a multitude of varying pI values. In agarose and polyacrylamide gels containing ampholytes, a linear pH gradient is generated when an electric field is applied. Each ampholyte molecule carries a net charge and will migrate within an electric field between the electrodes until it reaches the position corresponding to its pI, when it then stops moving to form a small plateau (stationary stack).