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PAGE reagents and buffers

Largely through its partner, SERVA, Scie-Plas provides a full range of products for gel polymerisation, sample preparation, continuous and discontinuous buffer systems, and protein staining and destaining following electrophoresis.


APS


A high speed initiator used in polyacrylamide gel electrophoresis.


Please click here for SERVA protocols on casting acry...

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TEMED

Catalyses the formation of free radicals by ammonium persulphate and accelerates the polymerisation of acrylamide and bis-acrylamide.


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Laemmli Electrophoresis Buffer (10x)

A tris-glycine/SDS electrophoresis buffer supplied as a 10x concentrate for SDS-PAGE. (0.25M Tris, 1.92M Glycine and 1% SDS in aqueous solution.



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Tris-Glycine/SDS Sample Buffer (2x)

A tris-glycine/SDS sample buffer supplied as a 2x concentrate. (Tris-HCl pH 6.8, 126mM, glycerol 20%, SDS 4%, bromophenol blue 0.02%).


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Tris-Tricine/SDS Buffer (10x)

A tris-tricine/SDS electrophoresis buffer supplied as a 10x concentrate for SDS-PAGE. (1M Tris, 1M Tricine and 1% SDS in aqueous solution).


Re...

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Tris-Tricine/SDS Sample Buffer (2x)

A tris-tricine/SDS sample buffer supplied as a 2x concentrate. (Tris-HCl pH 8.45, 90mM, Glycerol 24%, SDS 4%, SERVA Blue G 0.015%, Phenol Red 0.005%).



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Protein Marker

Ready to use for SDS-PAGE. Standard proteins ranging from 6.5 to 200 KDa. Protein content is approximately 0.15 to 0.3mg/ml.


The proteins included, in descending order of molec...

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Colloidal Coomassie Blue (CCB)

Coomassie Blue G250 is widely used to visualise proteins separated by agarose or acrylamide gel electrophoresis. This normally involves lengthy staining and destaining of gels using glacial acetic...

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Coomassie Blue Destaining Pads

CoZap™ is used for the rapid removal of Coomassie Blue stain from electrophoresis gels without the need to change the destaining solution. CoZap™ is a unique pad that ...

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